14° Congresso da Sociedade Latino Americana de Biomateriais, Orgãos Artificiais e Engenharia de Tecidos – SLABO
(5ª Edição do Workshop de Biomateriais, Engenharia de Tecidos e Orgãos Artificiais – OBI)
20 a 24 de Agosto de 2017 - Maresias - SP - Brasil

Mariano Grasselli, Currently Full Professor in the Universidad Nacional de Quilmes (UNQ), Argentina (1999 to date) and Principal Researcher of the National Scientific and Technical Research Council (CONICET) – Argentina (1999 to date). Director and founder of the Laboratory of Biotechnological Materials (UNQ- CONICET, 2010 to date). He holds a Bachelor's degree in Biochemistry (1989, Honor Diploma) and a Ph.D. in Organic Chemistry from the University of Buenos Aires (UBA, 1991). He has performed short postdoctoral studies in the field of Protein Biotechnology in Germany (1995) and Radiation Processing (RP) in Japan (Takasaki, 1998); Hungary (Budapest, 1999, 2000, 2001); France (Ecole polytechnique, 2003 / CEA-Saclay, 2004). He was Invited Scientist in Colombia, Brazil, Peru, France, Hungary, Poland and Germany in the field of protein purification and radiation processing. He was Invited expert in the field of ‘Preparation of nanomaterials by ionizing radiations’ (IAEA, 2013 and IPEN-Brazil, 2015). +60 published papers (+25 in RP), 5 patents (3 in RP, 2 International applications), 2 book chapters and 1 book. Reviewer of several journals in the field of RP and Biotechnology. He was advisor of 5 Ph.D. thesis and he was awarded with several grants form Argentina and International research projects such as EU-7PM, IAEA-CRP, BMBF, DAAD. He is a member of the Biophysics Society of Argentina. He has experience in the field of proteins, protein purification methods, materials for chromatography, track etched membranes and ionizing radiation methods (e-beam, gamma and X-rays source). Current interest is focused in development and nanostructuration of polymers and proteins by ionizing radiation methods.

 

HYBRID PROTEIN-GOLD NANOPARTICLES with bombesin FOR improving cell DELIVERY

Mariano Grasselli

Universidade Nacional de Quilmes, Argentina

Abstract
The use of nanotechnology in medicine, also called nanomedicine, is based on the preparation of nanostructures such as nanoparticles (NPs), with particular physicochemical characteristics which can be selected. They can be targeted to specific tumour tissues and modulate their pharmacokinetics, pharmacodynamics and enhanced intracellular activation properties. These characteristics depend on the size and surface properties of the NPs.
Albumin-based NPs can be designed for a variety of uses; among them, the ones that stand out are nanovehicles in a drug delivery system. Such choice relies in on the fact that Albumin is a protein with high biocompatibility, and is biodegradable and non–antigenic as well as malleability. Therefore, Albumin is an excellent material to be used for biocompatibility of inorganic materials such as gold NPs (Au-NPs). In addition, NP targeting for molecular recognition of anomalous cells could improve the drug delivery efficiency.
In this work are shown a preparation method of core/shell gold/Albumin NPs (Au/Alb-NPs) by desolvation method and crosslinking by ionizing radiation. Preparation of NPs containing an inorganic core such as gold can be very useful for NP tracking and detection. These Au/Alb-NPs are further decorated with a Bombesin-like peptide for prostate tumoral cell recognition.
Extensive characterization of the nanoconstructs has been done by LDS, FT-IR spectroscopy, zeta potential and AFM microscopy analysis. Hybrid NPs in the range of 60-80 nm have been achieved.
The nanostructured material was used to study the cellular uptake to PC3 prostate cancer cells. Multilayer Albumin coating shows higher cells uptake than the monolayer protein coated Au-NPs. Further studies should be done to study the cancer cell delivery in vivo.
These materials showed promising characteristics to be used as intracellular delivery carrier of antitumoral and/or diagnosis agents.

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Órgãos Artificiais e Biomecânica,Biomateriais,Normatização de Biomateriais,Liberação Controlada de Fármacos e Genes,Engenharia de Tecidos, Testes in vivo e in vitro, Medicina Regenerativa,Biopreservação,Materiais para Odontologia